A restricted access material for rapid analysis of [(11)C]-labeled radiopharmaceuticals and their metabolites in plasma

Nucl Med Biol. 2009 Nov;36(8):961-5. doi: 10.1016/j.nucmedbio.2009.07.004. Epub 2009 Oct 3.


Introduction: Analysis of the radioactive components in plasma taken during positron emission tomography (PET) measurements is often vital for the correct quantification of the PET data. The described high-performance liquid chromatography (HPLC) method has been developed to provide a fast, sensitive and robust method for the measurement of plasma samples from PET studies using [(11)C]-labeled radiopharmaceuticals.

Methods: Unadulterated plasma samples were analyzed directly, following a simple filtration, by the use of a small extraction column, containing a restricted access material, combined with a monolithic analysis column in a column-switching HPLC system.

Results: Up to 4 ml of plasma was analyzed by this method within 4.5-7 min in a fully automated process. Because of the rapid analysis, a large number of samples could be analyzed during a 90-min PET scan. The extraction column could be used for analysis of up to 500 ml of plasma before replacement was required.

Conclusions: The described method is fast and robust and the large sample volumes allow for accurate determination of the radioactive components in plasma even at 90 min after injection of a [(11)C]-labeled radiopharmaceutical.

Publication types

  • Evaluation Study

MeSH terms

  • Blood Chemical Analysis / methods*
  • Carbon Radioisotopes / blood*
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Radiopharmaceuticals / blood*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Ultrafiltration / methods*


  • Carbon Radioisotopes
  • Radiopharmaceuticals