[Changes of Neuropeptide Y and Leptin Levels in Rat Fed With Medium-Chain Triacylglycerides]

Wei Sheng Yan Jiu. 2009 Sep;38(5):538-41.
[Article in Chinese]


Objective: To investigate the effects of medium chain triglycerides (MCT) on adiposity and the modulation of neuropeptide Y (NPY) and leptin in Wistar rats.

Methods: 20 Wistar male rats were fed with diets containing 30% MCT or long chain triglycerides (LCT) for 8 weeks. Serum and hypothalamus leptin were detected by ELISA. RT-PCR was used to quantitatively compare the mRNA levels of recetpor of leptin (Ob-Rb) and NPY. The levels of serum and hypothalamic NPY were assessed by radioimmune assay.

Result: Body weight gains and body fat accumulations of rats fed with the MCT diets were more less than those of rats fed with the LCT diets (P < 0.01). Hypothalamus and serum NPY concentrations in rats fed with the MCT diets were more lower than those in rats fed with LCT diets (P < 0.01). Rats fed with MCT diets had significantly higher ratios of hypothalamus/serum leptin in comparition with rats fed with LCT diets. The Ob-Rb mRNA levels in the hypothalamus in the rats fed with the MCT diets were more higher than in those of rats fed with the LCT diets (P < 0.01). There were no significant differences in NPY expressions between the rats fed with the LCT diets and rats fed with MCT diets.

Conclusion: The MCT diet could decrease rats body weight gain by increasing Ob-Rb expression in hypothalamus and decreasing NPY level.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Dietary Fats / administration & dosage
  • Dietary Fats / pharmacology
  • Hypothalamus / metabolism
  • Leptin / blood
  • Leptin / genetics
  • Leptin / metabolism*
  • Male
  • Neuropeptide Y / genetics
  • Neuropeptide Y / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Receptors, Leptin / genetics
  • Receptors, Leptin / metabolism*
  • Triglycerides / administration & dosage
  • Triglycerides / pharmacology*
  • Weight Loss / drug effects*


  • Dietary Fats
  • Leptin
  • Neuropeptide Y
  • RNA, Messenger
  • Receptors, Leptin
  • Triglycerides