Haloechinothrix alba gen. nov., sp. nov., a halophilic, filamentous actinomycete of the suborder Pseudonocardineae

Int J Syst Evol Microbiol. 2010 Sep;60(Pt 9):2154-2158. doi: 10.1099/ijs.0.018531-0. Epub 2009 Oct 30.

Abstract

A novel halophilic, filamentous actinomycete strain, designated YIM 93221(T), was isolated from a salt lake in Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. The isolate grew with 9-23 % (w/v) NaCl and did not grow without NaCl. The isolate formed spiny aerial mycelium and did not form spores at maturity. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and glucose, glucosamine, mannose and an unknown sugar as the major whole-cell sugars. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unknown phospholipid. MK-8(H(4)) was the predominant menaquinone. The major fatty acid was iso-C(16 : 0). The DNA G+C content was 68.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 93221(T) formed a distinct lineage within the suborder Pseudonocardineae and showed 91.9-94.8 % 16S rRNA gene sequence similarity with members of the suborder Pseudonocardineae. On the basis of the evidence from this polyphasic study, a novel genus and species, Haloechinothrix alba gen. nov., sp. nov., are proposed. The type strain of Haloechinothrix alba is YIM 93221(T) (=DSM 45207(T) =CCTCC AB 208140(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / classification
  • Actinobacteria / genetics
  • Actinobacteria / isolation & purification
  • Actinobacteria / metabolism
  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification*
  • Actinomycetales / metabolism
  • Bacterial Typing Techniques
  • China
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Fatty Acids / metabolism
  • Fresh Water / microbiology*
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sodium Chloride / metabolism*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Sodium Chloride