Abstract
The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o-xylene pathway. Metabolite and reverse transcription-PCR analyses indicate that o-xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form cis-indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-indandiol undergoes ring cleavage by a meta-cleavage dioxygenase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / metabolism
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Biotransformation
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Gas Chromatography-Mass Spectrometry
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Gene Expression Profiling
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Hydroxylation
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Indans / metabolism*
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Magnetic Resonance Spectroscopy
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Metabolic Networks and Pathways
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Molecular Structure
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Oxidoreductases / metabolism
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RNA, Bacterial / genetics
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RNA, Messenger / genetics
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Rhodococcus / metabolism*
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Xylenes / metabolism*
Substances
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Bacterial Proteins
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Indans
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RNA, Bacterial
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RNA, Messenger
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Xylenes
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Oxidoreductases
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indan
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2-xylene