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Comparative Study
. 2010 Jan;76(1):352-5.
doi: 10.1128/AEM.02538-09. Epub 2009 Oct 30.

7-ketocholesterol Catabolism by Rhodococcus Jostii RHA1

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Free PMC article
Comparative Study

7-ketocholesterol Catabolism by Rhodococcus Jostii RHA1

Jacques M Mathieu et al. Appl Environ Microbiol. .
Free PMC article

Abstract

Oxysterols from steroid autooxidation have numerous harmful effects, but their biodegradation is poorly understood. Microarrays were used to study mineralization of the most common oxysterol, 7-ketocholesterol (7KC), by Rhodococcus jostii RHA1. Growth on 7KC versus growth on cholesterol resulted in 363 differentially expressed genes, including upregulation of two large gene clusters putatively encoding steroid catabolism. Despite this difference, 7KC degradation required key genes involved in cholesterol degradation, indicating a common catabolic route.

Figures

FIG. 1.
FIG. 1.
Gene clusters encoding enzymes involved in steroid catabolism in RHA1. (A) Locations of clusters (red) in the genome. (B) Genes with expression ratios greater than 2.0 on 7KC or cholesterol versus pyruvate.
FIG. 2.
FIG. 2.
Proposed scheme for 7KC metabolism based on metabolites accumulated by the RHA1 hsaC mutant. Degradation of the C-17 side chain, that of the 7-keto substituent, and that of rings A and B all appear to occur concurrently. R, side chain intact or in various possible stages of degradation; Red/SCD, 7-keto reductase plus partial or complete side chain degradation; Dht, dehydratase; 3,4,7-THSAP, 3,4,7-trihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione propionic acid; 3,4-DHSAP, 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione propionic acid; 3,4,7-THSA, 3,4,7-trihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione; 3,4-DHSA, 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione.

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