Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood

Neurosci Res. 2010 Feb;66(2):223-7. doi: 10.1016/j.neures.2009.10.007. Epub 2009 Oct 31.


Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CLOCK Proteins / genetics*
  • Cells, Cultured
  • Circadian Rhythm / genetics*
  • Gene Expression
  • Humans
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / physiology*
  • Male
  • Mice
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sleep Wake Disorders / genetics*
  • Sleep Wake Disorders / physiopathology*


  • RNA, Messenger
  • CLOCK Proteins