Core Protein of Chondroitin Sulfate Proteoglycan Promotes Neurite Outgrowth From Cultured Neocortical Neurons

J Neurochem. 1991 Feb;56(2):706-8. doi: 10.1111/j.1471-4159.1991.tb08207.x.

Abstract

Chondroitin sulfate proteoglycan (CS-PG) was purified from rat brain and examined for its effect on neurite outgrowth in primary cultures of embryonic rat neocortical neurons. Neurite outgrowth was increased in culture wells coated with CS-PG. The core protein and glycosaminoglycan (GAG) prepared from the CS-PG were also examined for neurite-promoting activity. The activity was observed in culture wells coated with the core protein but not with GAG. These results suggest that CS-PG stimulates neurite outgrowth from the cultured neurons via its core protein.

MeSH terms

  • Aggrecans
  • Animals
  • Axons / drug effects
  • Axons / physiology*
  • Brain Chemistry
  • Cells, Cultured
  • Cerebral Cortex / embryology
  • Cerebral Cortex / ultrastructure*
  • Chondroitin Sulfate Proteoglycans / analysis
  • Chondroitin Sulfate Proteoglycans / pharmacology*
  • Extracellular Matrix Proteins*
  • Glycoproteins / isolation & purification
  • Glycoproteins / pharmacology*
  • Glycosaminoglycans / pharmacology
  • Lectins, C-Type
  • Neurons / ultrastructure*
  • Proteoglycans*
  • Rats
  • Rats, Inbred Strains

Substances

  • Acan protein, rat
  • Aggrecans
  • Chondroitin Sulfate Proteoglycans
  • Extracellular Matrix Proteins
  • Glycoproteins
  • Glycosaminoglycans
  • Lectins, C-Type
  • Proteoglycans