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, Appendix 3, Appendix 3H

Differential Staining of Bacteria: Acid Fast Stain

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Differential Staining of Bacteria: Acid Fast Stain

Jackie Reynolds et al. Curr Protoc Microbiol.

Abstract

Acid-fastness is an uncommon characteristic shared by the genera Mycobacterium (Section 10A) and Nocardia. Because of this feature, this stain is extremely helpful in identification of these bacteria. Although Gram positive, acid-fast bacteria do not take the crystal violet into the wall well, appearing very light purple rather than the deep purple of normal Gram-positive bacteria.

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