Anti-obesity effect of Blumea balsamifera extract in 3T3-L1 preadipocytes and adipocytes

Am J Chin Med. 2009;37(5):843-54. doi: 10.1142/S0192415X09007326.

Abstract

Obesity, the leading metabolic disease in the world, is a serious health problem in industrialized countries. We investigated the anti-obesity effect of Blumea balsamifera extract on adipocyte differentiation of 3T3-L1 preadipocytes and anti-obesity effect of 3T3-L1 adipocytes. We found that treatment with an extract of Blumea balsamifera suppressed lipid accumulation and glycerol-3-phosphate dehydrogenase (GPDH) activity without affecting cell viability in 3T3-L1 preadipocytes and adipocytes. Furthermore, Blumea balsamifera extract brought significant attenuation of expressions of key adipogenic transcription factors, including peroxisome proliferator-activated receptor (PPAR)gamma, CCAAT element binding protein (C/EBPs) and leptin, however, induced up-regulation of adiponectin at the protein level in 3T3-L1 preadipocytes and adipocytes. These results suggest that Blumea balsamifera extract may block adipogenesis, at least in part, by decreasing key adipogenic transcription factors in 3T3-L1 preadipocytes and may have antiatherogenic, anti-inflammatory, and antidiabetic effects through up-regulation of adiponectin in 3T3-L1 adipocytes.

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology
  • Adipocytes / drug effects*
  • Adipocytes / metabolism
  • Adipogenesis / drug effects
  • Adiponectin / metabolism
  • Animals
  • Asteraceae / chemistry*
  • Azo Compounds / chemistry
  • Blotting, Western
  • CCAAT-Enhancer-Binding Protein-alpha / metabolism
  • CCAAT-Enhancer-Binding Protein-beta / metabolism
  • Cell Survival / drug effects
  • Glycerolphosphate Dehydrogenase / metabolism
  • Leptin / metabolism
  • Lipids / analysis
  • Mice
  • Obesity / prevention & control
  • PPAR gamma / metabolism
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry*
  • Staining and Labeling / methods

Substances

  • Adiponectin
  • Azo Compounds
  • CCAAT-Enhancer-Binding Protein-alpha
  • CCAAT-Enhancer-Binding Protein-beta
  • Leptin
  • Lipids
  • PPAR gamma
  • Plant Extracts
  • Glycerolphosphate Dehydrogenase
  • oil red O