Screening of specific diagnostic peptides of swine hepatitis E virus

Virol J. 2009 Nov 4;6:186. doi: 10.1186/1743-422X-6-186.

Abstract

Background: Swine hepatitis E virus (swHEV) is a zoonotic disease that is considered a major problem in pig production and presents a threat to human health. Elucidation of the major antigenic epitopes of swHEV is essential for the effective control of swHEV epidemics.

Results: By bioinformatic analysis, we identified and then synthesized 12 peptides from open reading frames (ORFs) ORF1, ORF2 and ORF3, including swHEV-1 - swHEV-12. Using the results from ELISA, we selected swHEV-11 as the best candidate antigen and used it as a coating antigen for the development of peptide-based swine anti-HEV ELISA kits. The coefficient of variation (CV) the coefficient of variation (CV) varied between 4.3-7.2% in the same batch, and between 8.2-17.7% in six different batches. When comparing our swine peptide-based kit with the commercial recombinant-based kit, the humane anti-HEV IgG test had a 73.4% correspondence rate for them.

Conclusion: This is the first systemic study to screen the diagnostic peptides of swHEV and our findings strongly suggest that peptide swHEV-11 is a potent diagnostic reagent of swHEV that could be used in the development of highly efficient diagnostic assays for the specific and highly sensitive detection of anti-HEV activity in swine serum samples.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Hepatitis Antigens / blood*
  • Hepatitis Antigens / chemistry
  • Hepatitis Antigens / immunology
  • Hepatitis E / diagnosis
  • Hepatitis E / immunology
  • Hepatitis E / veterinary*
  • Hepatitis E virus / chemistry
  • Hepatitis E virus / immunology*
  • Humans
  • Molecular Sequence Data
  • Open Reading Frames
  • Peptides / blood*
  • Peptides / chemistry
  • Peptides / immunology
  • Protein Structure, Secondary
  • Reproducibility of Results
  • Swine
  • Swine Diseases / blood*
  • Swine Diseases / diagnosis*
  • Swine Diseases / immunology

Substances

  • Hepatitis Antigens
  • Peptides