Abstract Honey samples from different floral sources were evaluated for their ability to inhibit the growth of 40 yeast strains (Candida albicans, C. krusei, C. glabrata and Trichosoporon spp.). Broth microdilution method (CLSI, M27-A2) was used to assess the activity of the honeys against yeasts at different concentrations ranging from 1.25-80% (v/v). All of the yeast strains tested were inhibited by honeys in this study. Broth microdilution assay revealed that inhibition of growth depends on the type and concentration of honey as well as the test pathogen. Little or no antifungal activity was seen at honey concentrations <2%. Rhododendron and multifloral honeys have generally more inhibitory effect than eucalyptus and orange honeys (P<0.05). Fluconazole-resistant yeast strains were examined for their susceptibility to honeys. This study demonstrated that, in vitro, these honeys had antifungal activity at the high concentration of 80% (v/v) in these fluconazole-resistant strains. Further studies are now required to demonstrate if this antifungal activity has any clinical application.