Effect of indoor air pollution from biomass fuel use on argyrophilic nuclear organizer regions in buccal epithelial cells

J Environ Pathol Toxicol Oncol. 2009;28(3):253-9. doi: 10.1615/jenvironpatholtoxicoloncol.v28.i3.70.


This study investigated the effect of indoor air pollution from biomass-fuel use on the expression of argyrophilic nucleolar organizer regions (AgNORs), an indicator of ribosome biosynthesis, in epithelial cells of oral mucosa. AgNORs were evaluated using cytochemical staining in 62 nonsmoking indian women (median age, 34 years), who cooked exclusively with biomass, and 55 age-matched women, who were from a similar neighborhood and cooked with relatively clean liquefied petroleum gas (LPG). Concentrations of particulate pollutants in indoor air were measured using a real-time aerosol monitor. Compared to the LPG-using controls, biomass-fuel users showed a remarkably increased number of AgNOR dots per nucleus (6.08 +/-2.26 vs 3.16 +/-0.86, p < 0.001), AgNOR size (0.85 +/-0.19 vs 0.53 +/-0.15 mum2, p < 0.001), and percentage of AgNOR-occupied nuclear area (4.88 +/-1.49 vs 1.75 +/-0.13%, p < 0.001). Biomass-using households had 2 to 4 times more particulate pollutants than that of LPG-using households. The changes in AgNOR expression were positively associated with PM10 and PM2.5 levels in indoor air after controlling for potential confounders such as age, kitchen location, and family income. Thus, biomass smoke appears to be a risk factor for abnormal cell growth via upregulation of ribosome biogenesis.

MeSH terms

  • Adult
  • Air Pollutants / adverse effects*
  • Air Pollution, Indoor / adverse effects*
  • Air Pollution, Indoor / analysis
  • Antigens, Nuclear / drug effects*
  • Biomass*
  • Cooking
  • Energy-Generating Resources
  • Female
  • Humans
  • Mouth Mucosa / drug effects*
  • Mouth Mucosa / pathology
  • Nucleolus Organizer Region / drug effects*
  • Silver Staining
  • Smoke / adverse effects*


  • Air Pollutants
  • Antigens, Nuclear
  • Smoke
  • nucleolar organizer region associated proteins