Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Feb;31(6):1307-15.
doi: 10.1016/j.biomaterials.2009.10.036. Epub 2009 Nov 4.

Nanoparticles Functionalized With Recombinant Single Chain Fv Antibody Fragments (scFv) for the Magnetic Resonance Imaging of Cancer Cells

Affiliations

Nanoparticles Functionalized With Recombinant Single Chain Fv Antibody Fragments (scFv) for the Magnetic Resonance Imaging of Cancer Cells

Kim L Vigor et al. Biomaterials. .

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) can substantially improve the sensitivity of magnetic resonance imaging (MRI). We propose that SPIONs could be used to target and image cancer cells if functionalized with recombinant single chain Fv antibody fragments (scFv). We tested our hypothesis by generating antibody-functionalized (abf) SPIONs using a scFv specific for carcinoembryonic antigen (CEA), an oncofoetal cell surface protein. SPIONs of different hydrodynamic diameter and surface chemistry were investigated and targeting was confirmed by ELISA, cellular iron uptake, confocal laser scanning microscopy (CLSM) and MRI. Results demonstrated that abf-SPIONs bound specifically to CEA-expressing human tumour cells, generating selective image contrast on MRI. In addition, we observed that the cellular interaction of the abf-SPIONs was influenced by hydrodynamic size and surface coating. The results indicate that abf-SPIONs have potential for cancer-specific MRI.

Similar articles

See all similar articles

Cited by 13 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback