TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins

J Biomol NMR. 2009 Dec;45(4):357-60. doi: 10.1007/s10858-009-9385-0. Epub 2009 Nov 5.

Abstract

A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of (15)N longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232-13237, 2007), by preserving the TROSY effect during the mixing period as well as the frequency encoding periods.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cadherins / chemistry
  • Humans
  • Kinetics
  • Magnetic Resonance Spectroscopy / methods*
  • Nitrogen Isotopes
  • Protein Structure, Tertiary
  • Proteins / chemistry*

Substances

  • CDH8 protein, human
  • Cadherins
  • Nitrogen Isotopes
  • Proteins