Parallel methods for expression and purification

Methods Enzymol. 2009;463:767-85. doi: 10.1016/S0076-6879(09)63041-X.

Abstract

Protein properties are highly diverse, making parallel expression and purification a particular challenge. Parallel methods are typically used when a number derivatives of a target protein are desired or when multiple homologs are needed. A typical scenario involves target evaluation, cloning and mutagenesis of the target, expression screening, large-scale expression and purification, and analytical and biophysical testing of the resulting protein. This chapter describes some of the strategies and methods employed for parallel protein expression and purification.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Fractionation / methods*
  • Cloning, Molecular / methods
  • Gene Expression* / physiology
  • Gene Transfer Techniques*
  • Genetic Vectors
  • High-Throughput Screening Assays / methods
  • Humans
  • Microchemistry / methods
  • Models, Biological
  • Recombinant Proteins / genetics*
  • Recombinant Proteins / isolation & purification*
  • Recombinant Proteins / metabolism

Substances

  • Recombinant Proteins