Accidental infection by Trypanosoma cruzi follow-up by the polymerase chain reaction: case report

Rev Inst Med Trop Sao Paulo. 2009 Sep-Oct;51(5):295-8. doi: 10.1590/s0036-46652009000500011.


We report a case of accidental infection by Trypanosoma cruzi in a 42-year-old female patient who presented an inoculation chagoma. Laboratory confirmation was based on examination of fresh blood, Giemsa-stained blood smear, immunoenzyme test (EIA-IgG), indirect immunofluorescence (IIF-IgM, IgG) and polymerase chain reaction (PCR). Only the PCR gave a positive result, and the EIA test was inconclusive. Two treatments with benznidazole were necessary. PCR was the only technique that continued to give positive results for approximately two months (65 days, or 2.2 months) following the second treatment and negative results from 96 days (3.2 months) to 850 days (28.3 months). We concluded that the presence of an inoculation chagoma and use of PCR were important and decisive for diagnosis and follow-up of the case.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Accidents, Occupational*
  • Adult
  • Animals
  • Chagas Disease / diagnosis
  • Chagas Disease / drug therapy
  • Chagas Disease / etiology*
  • Chagas Disease / transmission*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Hand Injuries / parasitology
  • Humans
  • Medical Laboratory Personnel*
  • Nitroimidazoles / therapeutic use
  • Polymerase Chain Reaction
  • Trypanocidal Agents / therapeutic use
  • Trypanosoma cruzi* / genetics
  • Trypanosoma cruzi* / immunology
  • Wounds, Stab / parasitology


  • Nitroimidazoles
  • Trypanocidal Agents
  • benzonidazole