The occurrence of matriptase C-terminal fragments on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells

Satoshi Tsuzuki; Yuka Miyake; Kuniyo Inouye; Tohru Fushiki

doi:10.1271/bbb.90431

The occurrence of matriptase C-terminal fragments on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells

Biosci Biotechnol Biochem. 2009 Nov;73(11):2538-40. doi: 10.1271/bbb.90431. Epub 2009 Nov 7.

Authors

Satoshi Tsuzuki¹, Yuka Miyake, Kuniyo Inouye, Tohru Fushiki

Affiliation

¹ Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan. tkmone@kais.kyoto-u.ac.jp

PMID: 19897900
DOI: 10.1271/bbb.90431

Abstract

Matriptase is a type II transmembrane serine protease. In the present study, matriptase C-terminal fragments containing the catalytic serine protease domain were found to occur on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells transfected with a cDNA encoding the protease. This suggests that matriptase interacts with various potential substrates when expressed in simple epithelia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Biocatalysis
Cell Line
Cell Membrane / metabolism
Cell Polarity*
Dogs
Epithelial Cells / cytology*
Humans
Molecular Sequence Data
Peptide Fragments / analysis*
Peptide Fragments / metabolism
Protein Structure, Tertiary
Rats
Serine Endopeptidases / chemistry*
Serine Endopeptidases / metabolism*

Substances

Peptide Fragments
Serine Endopeptidases
matriptase