Macrophage colony-stimulating factor activity in malignant pleural effusions. Its augmentation by intrapleural interleukin-2 infusions

Chest. 1991 Feb;99(2):377-81. doi: 10.1378/chest.99.2.377.


The activity of endogenous colony-stimulating factor (CSF) in malignant pleural effusions of lung cancer patients before and during daily intrapleural infusions of recombinant interleukin-2 (IL-2) was measured quantitatively by colony-forming bioassay and radioimmunoassay (RIA). Before therapy, malignant pleural effusions had various levels of CSF activities, and this CSF activity was neutralized almost completely by anti-M-CSF antibody. RIA also showed that the effusions contained various amounts of M-CSF. Daily intrapleural infusion of recombinant IL-2 caused significant increase in the CSF activities and M-CSF levels in pleural effusions. These results indicate that in vivo treatment with IL-2 induces production of endogenous M-CSF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Colony-Forming Units Assay
  • Humans
  • Interleukin-2 / therapeutic use*
  • Lung Neoplasms / complications
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / therapy
  • Macrophage Colony-Stimulating Factor / metabolism*
  • Middle Aged
  • Pleural Effusion, Malignant / etiology
  • Pleural Effusion, Malignant / metabolism*
  • Radioimmunoassay
  • Recombinant Proteins


  • Interleukin-2
  • Recombinant Proteins
  • Macrophage Colony-Stimulating Factor