Virucidal activity of 2 alcohol-based formulations proposed as hand rubs by the World Health Organization

Am J Infect Control. 2010 Feb;38(1):66-8. doi: 10.1016/j.ajic.2009.07.009. Epub 2009 Nov 8.


The virucidal activity of 2 hand rubs proposed by the World Health Organization was studied in a quantitative suspension test for chemical disinfectants and antiseptics in human medicine (EN 14476). These formulations are recommended if no hand rubs with declared microbiological activity are available in health care settings. Formulation I, based on ethanol, inactivated bovine viral diarrhea virus (BVDV), hepatitis C virus (HCV), adenovirus, and murine norovirus as a surrogate for human norovirus. Formulation II, based on isopropyl alcohol, was active only against adenovirus and enveloped viruses, such as BVDV and HCV. Both formulations failed to inactivate poliovirus by 4 log(10) steps within 300 seconds.

MeSH terms

  • Alcohols*
  • Disinfectants*
  • Hand Disinfection / methods*
  • Humans
  • Microbial Viability / drug effects*
  • Time Factors
  • Viruses / drug effects*
  • World Health Organization


  • Alcohols
  • Disinfectants