Detection of gene expression in embryonic tissues and stratified epidermis by in situ hybridization

Methods Mol Biol. 2010:585:253-60. doi: 10.1007/978-1-60761-380-0_17.

Abstract

Although recent molecular biology advances provide a very effective way in determining localized gene expression, the visualization of the expression by in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.

MeSH terms

  • Animals
  • Embryo, Mammalian / metabolism
  • Epidermis / metabolism*
  • Fixatives
  • Gene Expression*
  • In Situ Hybridization / methods*
  • Paraffin Embedding
  • RNA, Messenger / analysis*
  • RNA, Messenger / metabolism

Substances

  • Fixatives
  • RNA, Messenger