A nitric oxide releasing, self assembled peptide amphiphile matrix that mimics native endothelium for coating implantable cardiovascular devices

Biomaterials. 2010 Mar;31(7):1502-8. doi: 10.1016/j.biomaterials.2009.10.051. Epub 2009 Nov 12.


Cardiovascular disease is the number one cause of death in the United States. Deployment of stents and vascular grafts has been a major therapeutic method for treatment. However, restenosis, incomplete endothelialization, and thrombosis hamper the long term clinical success. As a solution to meet these current challenges, we have developed a native endothelial ECM mimicking self-assembled nanofibrous matrix to serve as a new treatment model. The nanofibrous matrix is formed by self-assembly of peptide amphiphiles (PAs), which contain nitric oxide (NO) donating residues, endothelial cell adhesive ligands composed of YIGSR peptide sequence, and enzyme-mediated degradable sites. NO was successfully released from the nanofibrous matrix rapidly within 48 h, followed by sustained release over period of 30 days. The NO releasing nanofibrous matrix demonstrated a significantly enhanced proliferation of endothelial cells (51+/-3% to 67+/-2%) but reduced proliferation of smooth muscle cells (35+/-2% to 16+/-3%) after 48 h of incubation. There was also a 150-fold decrease in platelet attachment on the NO releasing nanofibrous matrix (470+/-220 platelets/cm(2)) compared to the collagen-I (73+/-22 x 10(3)platelets/cm(2)) coated surface. The nanofibrous matrix has the potential to be applied to various cardiovascular implants as a self-assembled coating, thereby providing a native endothelial extracellular matrix (ECM) mimicking environment.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Aorta / cytology
  • Biomimetic Materials / pharmacology*
  • Blood Vessel Prosthesis
  • Blood Vessel Prosthesis Implantation
  • Cell Adhesion / drug effects
  • Cell Proliferation
  • Coated Materials, Biocompatible / pharmacology*
  • Collagen Type I / pharmacology
  • Endothelial Cells / cytology
  • Endothelial Cells / drug effects
  • Endothelium / drug effects*
  • Humans
  • Hydrogen-Ion Concentration / drug effects
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Myocytes, Smooth Muscle / cytology
  • Myocytes, Smooth Muscle / drug effects
  • Nanofibers / ultrastructure
  • Nitric Oxide / metabolism*
  • Peptides / chemistry
  • Peptides / pharmacology*
  • Platelet Adhesiveness / drug effects
  • Solvents
  • Stainless Steel / pharmacology
  • Surface-Active Agents / pharmacology*
  • Umbilical Veins / cytology


  • Coated Materials, Biocompatible
  • Collagen Type I
  • Peptides
  • Solvents
  • Surface-Active Agents
  • Stainless Steel
  • Nitric Oxide