Selective expression of the chemokine receptor XCR1 on cross-presenting dendritic cells determines cooperation with CD8+ T cells

Immunity. 2009 Nov 20;31(5):823-33. doi: 10.1016/j.immuni.2009.08.027. Epub 2009 Nov 12.


The expression of the chemokine receptor XCR1 and the function of its ligand XCL1 (otherwise referred to as ATAC, lymphotactin, or SCM-1) remained elusive to date. In the present report we demonstrated that XCR1 is exclusively expressed on murine CD8(+) dendritic cells (DCs) and showed that XCL1 is a potent and highly specific chemoattractant for this DC subset. CD8(+) T cells abundantly secreted XCL1 8-36 hr after antigen recognition on CD8(+) DCs in vivo, in a period in which stable T cell-DC interactions are known to occur. Functionally, XCL1 increased the pool of antigen-specific CD8(+) T cells and their capacity to secrete IFN-gamma. Absence of XCL1 impaired the development of cytotoxicity to antigens cross-presented by CD8(+) DCs. The XCL1-XCR1 axis thus emerges as an integral component in the development of efficient cytotoxic immunity in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / drug effects
  • CD8-Positive T-Lymphocytes / immunology*
  • Chemokines, C / pharmacology
  • Dendritic Cells / drug effects
  • Dendritic Cells / immunology*
  • Gene Expression Regulation / immunology*
  • Humans
  • Immunohistochemistry
  • Killer Cells, Natural / immunology
  • Mice
  • Mice, Knockout
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism*
  • Spleen / cytology


  • Chemokines, C
  • Receptors, G-Protein-Coupled
  • XCR1 protein, human
  • Xcl1 protein, mouse