Long-term Proliferating Early Pre B Cell Lines and Clones With the Potential to Develop to Surface Ig-positive, Mitogen Reactive B Cells in Vitro and in Vivo

EMBO J. 1991 Feb;10(2):327-36.

Abstract

Cell lines and clones were established from PB76-positive mouse fetal liver at day 13 and 14 of gestation, which proliferated with division times of a day in serum-substituted cultures under the stimulatory influence of adherent stromal cells and the cytokine IL-7 for periods longer than half a year. These lines expressed varying levels of the B lymphocyte lineage related markers PB76, B220, BP-1, VpreB and lambda 5, but no surface Ig or MHC class II molecules. All clones expressed PB76, VpreB and lambda 5 in a high percentage of cells, while B220 and/or BP-1 expression was low or undetectable in some. A cell line, and several clones established from it, all had kappa and lambda light chain genes in germ-line configuration. Either one or both of their H-chain-gene containing chromosomes carried a DH to JH. These pre B cell lines and clones could be induced to VH to DH and VL to JL rearrangements. This resulted in the development of varying percentages of sIg-positive surface, MHC class II negative, LPS-reactive B cells within 2-3 days, in the absence of contacts with stromal cells and/or IL-7. When injected into SCID mice, the cultured pre B cells populated the spleen of these mice to 5% with surface Ig-, MHC class II-positive LPS-reactive cells for greater than 25 weeks. The long-term in vitro proliferative capacity of these DH-JH rearranged pre B cell clones makes them major candidates for committed stem cells of the B lineage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Cell Differentiation
  • Cell Division / drug effects
  • Cell Line
  • Clone Cells
  • Female
  • Fetus
  • Gene Rearrangement
  • Genes, Immunoglobulin
  • Histocompatibility Antigens Class II / analysis
  • Immunologic Deficiency Syndromes / immunology
  • Interleukin-7 / pharmacology
  • Interleukins / pharmacology
  • Liver / immunology
  • Lymphocyte Activation*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Mice, Mutant Strains
  • Rats
  • Rats, Inbred Lew
  • Receptors, Antigen, B-Cell / analysis*
  • Recombinant Proteins / pharmacology
  • Spleen / immunology

Substances

  • Antibodies, Monoclonal
  • Histocompatibility Antigens Class II
  • Interleukin-7
  • Interleukins
  • Receptors, Antigen, B-Cell
  • Recombinant Proteins