Isolation of cDNAs partially encoding four Xenopus Wnt-1/int-1-related proteins and characterization of their transient expression during embryonic development

Dev Biol. 1991 Feb;143(2):230-4. doi: 10.1016/0012-1606(91)90073-c.

Abstract

To begin to study the functions of the Wnt-1/int-1 gene family during vertebrate development, we have isolated four Xenopus laevis cDNAs encoding the partial sequence of proteins homologous to Wnt-1/int-1. Xwnt-3, Xwnt-4, Xwnt-5A, and Xwnt-8 demonstrate between 35 and 50% amino acid identity with X. laevis Wnt-1/int-1 and most cysteine residues are conserved. Xwnt-4 and Xwnt-3 transcripts are detected only during the neurula through tadpole stages of development. Expression of Xwnt-8 is observable during gastrulation, declines during neurulation, and is undetectable by the tadpole stage of development. Xwnt-5A transcripts are most prevalent in RNA from oocytes and tadpoles, although low level expression is detected at all stages examined. The temporal changes in expression of these transcripts imply a unique role for each Xwnt during embryogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Proto-Oncogene Proteins / genetics*
  • RNA, Messenger / genetics
  • Wnt Proteins
  • Wnt1 Protein
  • Xenopus Proteins
  • Xenopus laevis / embryology
  • Xenopus laevis / genetics*
  • Zebrafish Proteins*

Substances

  • Oligonucleotides
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • WNT1 protein, Xenopus
  • Wnt Proteins
  • Wnt1 Protein
  • Xenopus Proteins
  • Zebrafish Proteins
  • wnt8a protein, Xenopus
  • DNA

Associated data

  • GENBANK/M55054
  • GENBANK/M55055
  • GENBANK/M55056
  • GENBANK/M55058
  • GENBANK/M65223
  • GENBANK/M65224
  • GENBANK/S60919
  • GENBANK/S60920
  • GENBANK/S89458
  • GENBANK/X59082