Novel human papillomavirus type 18 replicon and its application in screening the antiviral effects of cytokines

Cancer Sci. 2010 Feb;101(2):536-42. doi: 10.1111/j.1349-7006.2009.01411.x. Epub 2009 Oct 22.


Human papillomaviruses (HPVs) infect the stratified epithelial organ. The infection induces benign tumors, which occasionally progress into malignant tumors. To elucidate the virus-induced tumorigenesis, an understanding of the lifecycle of HPV is crucial. In this report, we developed a new system for the analysis of the HPV lifecycle. The new system consists of a novel HPV replicon and an organotypic "raft" culture, by which the HPV-DNA is maintained stably in normal human keratinocytes for a long period and the viral vegetative replication is reproduced. This system will benefit biochemical and genetic studies on the lifecycle of HPV and tumorigenesis. This system is also valuable in screening for antiviral compounds. We confirmed its usefulness by evaluating the antivirus effect of cytokines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / pharmacology*
  • Cells, Cultured
  • Cytokines / pharmacology*
  • DNA, Viral / analysis
  • Human papillomavirus 18 / drug effects*
  • Human papillomavirus 18 / genetics
  • Humans
  • Replicon / drug effects*
  • Virus Replication / drug effects


  • Antiviral Agents
  • Cytokines
  • DNA, Viral