Panel of prototypical raltegravir-resistant infectious molecular clones in a novel integrase-deleted cloning vector

Antimicrob Agents Chemother. 2010 Feb;54(2):934-6. doi: 10.1128/AAC.01345-09. Epub 2009 Nov 16.


We created an HIV-1 cloning vector, pNL4.3DeltaIN, to generate recombinant infectious molecular clones for analysis of patient-derived HIV-1 integrase coding regions. Using this vector, we constructed a panel of clinically derived viruses with the canonical patterns of raltegravir resistance mutations and submitted the panel to the NIH AIDS Research and Reference Reagent Program. Investigational integrase inhibitors with activity against these clones are likely to retain activity against the most clinically relevant raltegravir-resistant variants.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Drug Resistance, Viral / genetics*
  • HIV Infections / virology
  • HIV Integrase / genetics*
  • HIV Integrase Inhibitors / pharmacology*
  • HIV-1 / drug effects*
  • HIV-1 / genetics*
  • HIV-1 / physiology
  • Humans
  • Molecular Sequence Data
  • Mutation
  • Pyrrolidinones / pharmacology*
  • Raltegravir Potassium


  • HIV Integrase Inhibitors
  • Pyrrolidinones
  • Raltegravir Potassium
  • HIV Integrase
  • p31 integrase protein, Human immunodeficiency virus 1

Associated data

  • GENBANK/GU076499
  • GENBANK/GU076502
  • GENBANK/GU076504
  • GENBANK/GU076505
  • GENBANK/GU076506
  • GENBANK/GU076507
  • GENBANK/GU076508
  • GENBANK/GU076510
  • GENBANK/GU076511
  • GENBANK/GU076512