Abstract
Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His(6)-tagged fusion proteins in E. coli. The fusion proteins were isolated and shown to bind beta-lactams, indicating these putative PBPs have penicillin-binding activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism*
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Burkholderia / enzymology*
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Cloning, Molecular
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Escherichia coli / genetics
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Gene Expression
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Humans
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Penicillin-Binding Proteins / genetics*
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Penicillin-Binding Proteins / metabolism*
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Protein Binding
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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beta-Lactams / metabolism*
Substances
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Bacterial Proteins
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Penicillin-Binding Proteins
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Recombinant Fusion Proteins
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beta-Lactams