A tribute to Dr. David Kline: a new approach to an old peripheral nerve problem--splicing instead of regenerating disrupted axons

Neurosurgery. 2009 Oct;65(4 Suppl):A52-4. doi: 10.1227/01.NEU.0000335655.66073.27.

Abstract

Objective: Our goal is to develop a novel method to repair damaged axons. This method relies on acutely restoring axonal continuity rather than the traditional approach of promoting axonal regeneration.

Methods: Micro- and nanoechnological methods, in combination with focal application of electrical fields, are applied to individual and groups of axons both in vitro and in vivo.

Results: Application of these techniques has permitted micromanipulation of axons at the cellular level and fusion of axonal membranes.

Conclusion: Although a great deal more work is necessary, our findings suggest that it may one day be possible to repair acutely disrupted axons by splicing their membranes back together.

Publication types

  • Review

MeSH terms

  • Axons / physiology
  • Axons / radiation effects
  • Axons / ultrastructure
  • Cell Membrane / physiology
  • Cell Membrane / ultrastructure
  • Electric Stimulation Therapy / methods
  • Electromagnetic Fields
  • Humans
  • Microsurgery / methods
  • Nanotechnology / methods
  • Nanotechnology / trends
  • Nerve Regeneration / physiology*
  • Nerve Regeneration / radiation effects
  • Neurosurgical Procedures / methods*
  • Peripheral Nerves / pathology
  • Peripheral Nerves / physiopathology
  • Peripheral Nerves / surgery*
  • Peripheral Nervous System Diseases / pathology
  • Peripheral Nervous System Diseases / physiopathology
  • Peripheral Nervous System Diseases / surgery*
  • Recovery of Function / physiology
  • Recovery of Function / radiation effects