Improved spatial resolution in fluorescence focal modulation microscopy

Opt Lett. 2009 Nov 15;34(22):3508-10. doi: 10.1364/OL.34.003508.

Abstract

We show that the focal modulation microscopy (FMM), which combines a spatial phase modulator with confocal microscopy, results in an improvement in spatial resolution. This technique was introduced to increase imaging depth into tissue and rejection of background from a thick scattering object. A theory for image formation in FMM is presented, and the effects of detecting the in-phase modulated fluorescence signal are discussed. Compared with conventional confocal microscopy, the width of the point-spread function for the in-phase fluorescence signal is improved by 16.4%. When applied to saturable fluorescence, the half-width at half-maximum is improved by 33.6%, 50.0%, and 62.9%, at demodulation frequencies 2omega, 4omega, and 8omega, respectively.

MeSH terms

  • Microscopy, Confocal
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*