A peptide tag system for facile purification and single-molecule immobilization

Biochemistry. 2009 Dec 22;48(50):11834-6. doi: 10.1021/bi901756n.


A peptide fusion tag and accompanying recombinant capture reagents have been developed on the basis of the peptide-PDZ domain interaction and affinity clamps, a new class of affinity reagent. This system allows for single-step purification under mild conditions and stable capture of a tagged protein. The subnanomolar affinity, high force resistance (>30 pN), small size ( approximately 25 kDa, approximately one-sixth of the size of IgG), and monomeric nature of the affinity clamp are all superior features for many applications, in particular single-molecule measurements.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Affinity Labels
  • Antibodies, Monoclonal / chemistry
  • Biotin / chemistry
  • Humans
  • Indicators and Reagents
  • Microscopy, Fluorescence
  • Myosins / chemistry*
  • Myosins / isolation & purification
  • Peptide Fragments / chemistry*
  • Peptide Fragments / genetics
  • Peptide Fragments / isolation & purification
  • Protein Engineering
  • Recombinant Fusion Proteins / chemistry
  • Streptavidin / chemistry


  • Affinity Labels
  • Antibodies, Monoclonal
  • Indicators and Reagents
  • Peptide Fragments
  • Recombinant Fusion Proteins
  • Biotin
  • Streptavidin
  • Myosins