Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate hydroxylase gene (nahG) and its 3'-flanking region

Biochemistry. 1991 Feb 12;30(6):1635-41. doi: 10.1021/bi00220a028.


Gene nahG of naphthalene/salicylate catabolic plasmid NAH7 encodes a protein of molecular weight 45,000, salicylate hydroxylase. This enzyme catalyzes the formation of catechol from salicylate, a key intermediate in naphthalene catabolism. DNA sequence analysis of the 3.1-kilobase HindIII fragment containing the nahG locus reveals an open reading frame (ORF) of 1305 base pairs that corresponds to a protein of 434 amino acid residues. The predicted amino acid sequence of salicylate hydroxylase is in agreement with the molecular weight, NH2-terminal amino acid sequence, and total amino acid composition of the purified salicylate hydroxylase [You, I.-S., Murray, R. I., Jollie, D., & Gunsalus, I. C. (1990) Biochem. Biophys. Res. Commun. 169, 1049-1054]. The amino acid sequence between positions 8 and 37 of salicylate hydroxylase shows homology with known ADP binding sites of other FAD-containing oxidoreductases, thus confirming its biochemical function. The sequence of the Pseudomonas putida salicylate hydroxylase was compared with those of other similar flavoproteins. A small DNA segment (831 base pairs) disrupts the continuity of the known gene order nahG and nahH, the latter encoding catechol 2,3-dioxygenase. The complete nucleotide sequence of the intergenic region spanning genes nahG and nahH has been determined and its biological role proposed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Genes, Bacterial*
  • Mixed Function Oxygenases / genetics*
  • Molecular Sequence Data
  • Molecular Weight
  • Open Reading Frames*
  • Pseudomonas / enzymology
  • Pseudomonas / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid


  • Mixed Function Oxygenases
  • salicylate 1-monooxygenase

Associated data

  • GENBANK/J05317
  • GENBANK/M60055
  • GENBANK/M62346
  • GENBANK/M62347
  • GENBANK/M62348
  • GENBANK/M62349
  • GENBANK/M62350
  • GENBANK/M62351
  • GENBANK/M62352
  • GENBANK/M62353
  • GENBANK/M62354