Abstract
Lactobacillus plantarum ctsR was characterized. ctsR was found to be cotranscribed with clpC and induced in response to various abiotic stresses. ctsR deletion conferred a heat-sensitive phenotype with peculiar cell morphological features. The transcriptional pattern of putative CtsR regulon genes was examined in the Delta ctsR mutant. Direct CtsR-dependent regulation was demonstrated by DNA-binding assays using recombinant CtsR and the promoters of the ctsR-clpC operon and hsp1.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism*
-
Bacterial Proteins / physiology*
-
Gene Expression Regulation, Bacterial / genetics
-
Gene Expression Regulation, Bacterial / physiology
-
Heat-Shock Proteins
-
Lactobacillus plantarum / genetics
-
Lactobacillus plantarum / metabolism*
-
Lactobacillus plantarum / ultrastructure
-
Microscopy, Atomic Force
-
Promoter Regions, Genetic / genetics
-
Protein Binding
-
Regulon / genetics
-
Regulon / physiology*
-
Repressor Proteins / genetics
-
Repressor Proteins / metabolism*
-
Repressor Proteins / physiology*
-
Reverse Transcriptase Polymerase Chain Reaction
-
Temperature
Substances
-
Bacterial Proteins
-
ClpC protein, Bacteria
-
CtsR protein, bacteria
-
Heat-Shock Proteins
-
Repressor Proteins