Actinokineospora baliensis sp. nov., Actinokineospora cibodasensis sp. nov. and Actinokineospora cianjurensis sp. nov., isolated from soil and plant litter

Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2331-2335. doi: 10.1099/ijs.0.013276-0. Epub 2009 Nov 20.


Six actinomycete strains isolated from soil and plant-litter samples in Indonesia were studied for their taxonomic position by using a polyphasic approach. Phylogenetically, all the strains were located in the broad cluster of the genus Actinokineospora. Chemotaxonomic data [cell-wall diamino acid, meso-diaminopimelic acid; cell-wall peptidoglycan, type III (A1γ); major sugars, galactose and arabinose; major menaquinone, MK-9(H₄); major fatty acid, iso-C₁₆:₀; major phospholipid, phosphatidylethanolamine] supported the affiliation of all six strains to the genus Actinokineospora. The results of DNA-DNA hybridization with DNA from type strains of Actinokineospora species with validly published names revealed three DNA-DNA relatedness groups. Group I (ID03-0561(T)) showed low relatedness to the other strains studied. The three strains in group II (ID03-0784(T), ID03-0808 and ID03-0809) formed a group with high relatedness (98-100 %) and showed low relatedness to the other strains studied. The two strains in group III (ID03-0810(T) and ID03-0813) showed 58-68 % relatedness to Actinokineospora terrae NBRC 15668(T) and showed low relatedness (2-24 %) to the other strains studied. The description of three novel species is proposed: Actinokineospora baliensis sp. nov., for the single strain in group I (type strain ID03-0561(T) =BTCC B-554(T) =NBRC 104211(T)), Actinokineospora cibodasensis sp. nov., for the strains in group II (type strain ID03-0784(T) =BTCC B-555(T) =NBRC 104212(T)), and Actinokineospora cianjurensis sp. nov., for the strains in group III (type strain ID03-0810(T) =BTCC B-558(T) =NBRC 105526(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / chemistry
  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification*
  • Carbohydrates / analysis
  • Cell Wall / chemistry
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Diaminopimelic Acid / analysis
  • Fatty Acids / analysis
  • Indonesia
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Peptidoglycan / analysis
  • Phospholipids / analysis
  • Phylogeny
  • Plants / microbiology*
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*


  • Carbohydrates
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Peptidoglycan
  • Phospholipids
  • Quinones
  • RNA, Ribosomal, 16S
  • Diaminopimelic Acid

Associated data

  • GENBANK/AB447488
  • GENBANK/AB447489
  • GENBANK/AB473945