The Late Upper Paleolithic burial Villabruna 1 (Val Cismon, Belluno, Italy), directly dated to about 14,000 years ago (calibrated chronology), includes a well preserved skeleton accompanied by grave goods and covered with painted stones. The skeleton belongs to an adult male, about twenty-five years old, characterized by a relatively tall stature for the time period, short trunk and more linear body proportions than its contemporaries, similar to those of recent North-African populations. Multivariate statistical analysis of craniofacial characteristics place Villabruna 1 close to Le Bichon 1, a geographically and chronologically nearby specimen, suggesting genetic affinity among the last hunter and gatherers from the alpine region. Observations on dental wear and microwear indicate that the anterior dentition was involved in non-alimentary activities such as the mastication of fibrous materials including large abrasive contaminants. Whereas the information on dietary habits drawn from dental wear is not conclusive, stable isotopes analysis points to a terrestrially based diet rich in animal proteins. Biomechanical study of major long bones indicates heightened overall robusticity and marked humeral asymmetry. These results suggest intense unimanual activity, possibly linked to repeated throwing movements in hunting, and the combined effect of mobile lifestyle and mountainous terrain, as far as the femur is concerned. Paleopathological analysis did not reveal signs of any major event which might help identify a possible cause of death. However, macroscopic and radiographic examination of the skull reveals traces of porotic hyperostosis, indicative of a healed anemic condition. Finally, localized tibial periostitis, probably of traumatic origin, and lumbar hyperlordosis associated with deformations of vertebral bodies and L5 spondylolysis provide evidence of additional, minor, pathological changes.