Utilizing all forms of sugars derived from lignocellulosic biomass via various pretreatment and hydrolysis process is a primary criterion for selecting a microorganism to produce biofuels and biochemicals. A broad carbon spectra and potential inhibitors such as furan, phenol compounds and weak acids are two major obstacles that limited the application of dilute-acid hydrolysate of lignocellulosics in lactic acid fermentation. Two strains of bacteria isolated from sour cabbage, S3F4 (Lactobacillus brevis) and XS1T3-4 (Lactobacillus plantrum), exhibited the ability to utilize various sugars present in dilute-acid hydrolysate of biomass. The S3F4 strain also showed strong resistance to potential fermentation inhibitors such as ferulic acid and furfural. Fermentation in flasks by this strain resulted in 39.1 g/l of lactic acid from dilute acid hydrolysates of corncobs that had initial total sugar concentration of 56.9 g/l (xylose, 46.4 g/l; glucose, 4.0 g/l; arabinose, 6.5 g/l). The hydrolysate of corncobs was readily utilized by S3F4 without detoxification, and the lactic acid concentration obtained in this study was higher compared to other reports.