Expression of leptin receptor gene in developing and adult zebrafish

Gen Comp Endocrinol. 2010 Apr 1;166(2):346-55. doi: 10.1016/j.ygcen.2009.11.015. Epub 2009 Nov 24.


Interactions of leptin and leptin receptors play crucial roles during animal development and regulation of appetite and energy balance. In this study we analyzed expression pattern of a zebrafish leptin receptor gene in both developing and adult zebrafish using in situ hybridization and Q-PCR methods. Zebrafish leptin receptor message (lepr) was detected in all embryonic and larval stages examined, and in adult zebrafish. In embryonic zebrafish, lepr was mainly expressed in the notochord. As development proceeded, lepr expression in the notochord decreased, while its expression in several other tissues, including the trunk muscles and gut, became evident. In both larval and adult brains, large lepr expressing cells were detected in similar regions of the hindbrain. In adult zebrafish, lepr expression was also observed in several other brain regions including the hypothalamic lateral tuberal nucleus, the fish homolog of the arcuate nucleus. Q-PCR experiments confirmed lepr expression in the adult fish brain, and also showed lepr expression in several adult tissues including liver, muscle and gonads. Our results showed that lepr expression was both spatially and temporally regulated.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / metabolism
  • Gene Expression Regulation
  • Gene Expression*
  • Gonads / metabolism
  • In Situ Hybridization
  • Larva / metabolism
  • Liver / metabolism
  • Molecular Sequence Data
  • Muscles / metabolism
  • Notochord / metabolism
  • Organ Specificity
  • Receptors, Leptin / chemistry
  • Receptors, Leptin / genetics*
  • Sequence Alignment
  • Zebrafish / embryology
  • Zebrafish / growth & development*
  • Zebrafish / metabolism*


  • Receptors, Leptin