Large-scale purification of membrane-containing bacteriophage PRD1 and its subviral particles

Virology. 1991 Mar;181(1):348-52. doi: 10.1016/0042-6822(91)90501-2.

Abstract

PRD1 is a dsDNA virus that infects Escherichia coli and Salmonella typhimurium. The genome is a linear molecule with 5' covalently linked terminal protein. The virus has a lipid membrane inside the protein coat. We describe the large-scale purification of the virus using a zonal rotor and the yields and quality of the virus and its subviral assemblies for subsequent biophysical measurements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Centrifugation, Zonal
  • DNA, Viral / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel
  • Salmonella Phages / isolation & purification*
  • Salmonella typhimurium

Substances

  • DNA, Viral