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, 1 (2), 207-215

Simultaneous Imaging of a lacZ-marked Tumor and Microvasculature Morphology in Vivo by Dual-Wavelength Photoacoustic Microscopy

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Simultaneous Imaging of a lacZ-marked Tumor and Microvasculature Morphology in Vivo by Dual-Wavelength Photoacoustic Microscopy

Li Li et al. J Innov Opt Health Sci.

Abstract

Photoacoustic molecular imaging, combined with the reporter-gene technique, can provide a valuable tool for cancer research. The expression of the lacZ reporter gene can be imaged using photoacoustic imaging following the injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme β-galactosidase. Dual-wavelength photoacoustic microscopy was used to non-invasively image the detailed morphology of a lacZ-marked 9L gliosarcoma and its surrounding microvasculature simultaneously in vivo, with a superior resolution on the order of 10 μm. Tumor-feeding vessels were found, and the expression level of lacZ in tumor was estimated. With future development of new absorption-enhancing reporter-gene systems, we anticipate this strategy can lead to a better understanding of the role of tumor metabolism in cancer initiation, progression, and metastasis, and in its response to therapy.

Figures

Figure 1
Figure 1
Schematic of the dual-wavelength photoacoustic microscopy system.
Figure 2
Figure 2
Comparison of the molar extinction spectra of the blue product, HbO2, and HbR.
Figure 3
Figure 3
In-vivo images of lacZ-marked tumor by dual-wavelength photoacoustic microscopy. (A) MAP mage acquired at 635 nm showing tumor morphology. (B) MAP image acquired at 584 nm showing microvasculature, or the spatial distribution of total hemoglobin concentration. (C) Combined pseudo-colored image showing the spatial relations between tumor and vascular network. Red: Blood vessels. Blue: tumor. Arrows indicate feeding vessels of tumor.

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