Identification of a brain-specific 27/26-kDa extracellular protein with the monoclonal antibody to differentiated PC12h pheochromocytoma cells

M Ishikawa; Y Kuroda; K Kobayashi; H Sawada; M Hayashi

doi:10.1016/0014-4827(91)90549-a

Identification of a brain-specific 27/26-kDa extracellular protein with the monoclonal antibody to differentiated PC12h pheochromocytoma cells

Exp Cell Res. 1991 Mar;193(1):151-4. doi: 10.1016/0014-4827(91)90549-a.

Authors

M Ishikawa¹, Y Kuroda, K Kobayashi, H Sawada, M Hayashi

Affiliation

¹ Department of Biology, Ochanomizu University, Tokyo, Japan.

PMID: 1995290
DOI: 10.1016/0014-4827(91)90549-a

Abstract

We have searched for brain-specific extracellular molecules using a library of monoclonal antibodies against surface antigens of differentiated PC12h cells. One of the monoclonal antibodies, PCH42-14, recognized a 27/26-kDa protein of 10-week-old rat brain on immunoblotting. PCH42-14 antigen was detected only in brain, especially in cerebrum, olfactory bulb, mesencephalon, hippocampus, medulla oblongata, and spinal cord. On hippocampal neuron culture, PCH42-14 antigen existed extracellularly along with the neuronal extensions.

MeSH terms

Animals
Antibodies, Monoclonal
Antigens, Surface / analysis*
Brain Chemistry*
Immunoblotting
Molecular Weight
Nerve Tissue Proteins / analysis*
Neurons / chemistry*
Organ Specificity
Pheochromocytoma
Rats
Rats, Inbred Strains
Tumor Cells, Cultured

Substances

Antibodies, Monoclonal
Antigens, Surface
Nerve Tissue Proteins