Probiotic Lactic Acid Bacterium From Kanjika as a Potential Source of Vitamin B12: Evidence From LC-MS, Immunological and Microbiological Techniques

Biotechnol Lett. 2010 Apr;32(4):503-6. doi: 10.1007/s10529-009-0176-1. Epub 2009 Dec 2.

Abstract

Vitamin B(12) was produced by probiotic Lactobacillus plantarum in submerged fermentation (96 h) with successive anaerobic and aerobic phases of 48 h each to give 13 ng vitamin B(12)/g dry biomass. Sodium cyanide-mediated cell lysis, followed by benzyl alcohol/chloroform/water extraction, improved the release of intracellular vitamin B(12) for analysis. The presence of the K(+) adduct of cyanocobalamin (m/z of 1394) was established using electron spray ionization-mass spectra; growth of a mutant of Escherichia coli in the presence of cyanocobalamin ascertained its bioavailability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Anaerobiosis
  • Chlorides / pharmacology
  • Chromatography, High Pressure Liquid
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli / growth & development
  • Escherichia coli / metabolism
  • Fermentation
  • Food Microbiology
  • Lactobacillus plantarum / drug effects
  • Lactobacillus plantarum / isolation & purification
  • Lactobacillus plantarum / metabolism*
  • Mass Spectrometry
  • Probiotics / metabolism*
  • Time Factors
  • Vitamin B 12 / biosynthesis*
  • Zinc Compounds / pharmacology

Substances

  • Chlorides
  • Zinc Compounds
  • zinc chloride
  • Vitamin B 12