Human colon carcinoma, fibrosarcoma and leukemia cell lines produce tumor-associated trypsinogen

Int J Cancer. 1991 Feb 20;47(4):592-6. doi: 10.1002/ijc.2910470419.


Previous studies have indicated that cyst fluid of ovarian tumors contains 2 trypsinogen isoenzymes, called tumor-associated trypsinogen-I (TAT-I) and trypsinogen-2 (TAT-2), the levels of which correlate with the degree of malignancy of the tumors. In addition, these cyst fluids contain large amounts of tumor-associated trypsin inhibitor (TATI), which is also expressed in many other human tumors. In the present study we examined the production of TAT-I, TAT-2 and TATI in 9 established tumor-cell lines. TAT-2 was produced by 5 cell lines. Its concentration in the conditioned medium of COLO 205 colon adenocarcinoma cells, K-562 erythroleukemia cells and fibrosarcoma cell lines HT 1080, 8387 and A 9733 was 460 micrograms/l, 9.8 micrograms/l, 21 micrograms/l, 8.8 micrograms/l and 0.24 micrograms/l, respectively. TAT-I was detectable in the conditioned medium of COLO 205 and HT 1080 cells at concentrations of 64 micrograms/l and 0.5 micrograms/l, respectively. TATI was detected only in the media of COLO 205 cells at a concentration of 23 micrograms/l. TAT-2 zymogen was purified from the conditioned medium of COLO 205 and HT 1080 cells by immunoaffinity chromatography. According to its aminoterminal amino acid sequence, a molecular mass of 28 kDa by SDS-PAGE, elution pattern in ion-exchange chromatography and ability to be activated by enteropeptidase, the zymogen is identical to that previously isolated from cyst fluid of ovarian tumors. In addition, we found that TAT-2 secretion could be down-regulated by dexamethasone in HT 1080 cells but not in COLO 205 cells. The abundant production of TAT-2 isoenzyme in different cancer cell lines suggests that it could contribute to the increased proteolytic activity of many human tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma / enzymology*
  • Colonic Neoplasms / enzymology*
  • Dexamethasone / pharmacology
  • Extracellular Matrix / metabolism
  • Fibrosarcoma / enzymology*
  • Humans
  • Isoenzymes / analysis*
  • Leukemia / enzymology*
  • Trypsinogen / biosynthesis*
  • Trypsinogen / isolation & purification
  • Trypsinogen / physiology
  • Tumor Cells, Cultured


  • Isoenzymes
  • Dexamethasone
  • Trypsinogen