Epigenetic silencing in Friedreich ataxia is associated with depletion of CTCF (CCCTC-binding factor) and antisense transcription

PLoS One. 2009 Nov 19;4(11):e7914. doi: 10.1371/journal.pone.0007914.

Abstract

Background: Over 15 inherited diseases are caused by expansion of triplet-repeats. Friedreich ataxia (FRDA) patients are homozygous for an expanded GAA triplet-repeat sequence in intron 1 of the FXN gene. The expanded GAA triplet-repeat results in deficiency of FXN gene transcription, which is reversed via administration of histone deacetylase inhibitors indicating that transcriptional silencing is at least partially due to an epigenetic abnormality.

Methodology/principal findings: We found a severe depletion of the chromatin insulator protein CTCF (CCCTC-binding factor) in the 5'UTR of the FXN gene in FRDA, and coincident heterochromatin formation involving the +1 nucleosome via enrichment of H3K9me3 and recruitment of heterochromatin protein 1. We identified FAST-1 (FXNAntisense Transcript - 1), a novel antisense transcript that overlaps the CTCF binding site in the 5'UTR, which was expressed at higher levels in FRDA. The reciprocal relationship of deficient FXN transcript and higher levels of FAST-1 seen in FRDA was reproduced in normal cells via knockdown of CTCF.

Conclusions/significance: CTCF depletion constitutes an epigenetic switch that results in increased antisense transcription, heterochromatin formation and transcriptional deficiency in FRDA. These findings provide a mechanistic basis for the transcriptional silencing of the FXN gene in FRDA, and broaden our understanding of disease pathogenesis in triplet-repeat diseases.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions
  • Alleles
  • CCCTC-Binding Factor
  • Epigenesis, Genetic*
  • Fibroblasts / metabolism
  • Forkhead Transcription Factors / metabolism
  • Friedreich Ataxia / genetics*
  • Gene Expression Profiling
  • Gene Silencing*
  • Heterochromatin / metabolism
  • Humans
  • Iron-Binding Proteins / physiology*
  • Oligonucleotides, Antisense / metabolism
  • Repressor Proteins / genetics*
  • Repressor Proteins / physiology*
  • Transcription, Genetic
  • Zinc Fingers

Substances

  • 5' Untranslated Regions
  • CCCTC-Binding Factor
  • CTCF protein, human
  • FOXH1 protein, human
  • Forkhead Transcription Factors
  • Heterochromatin
  • Iron-Binding Proteins
  • Oligonucleotides, Antisense
  • Repressor Proteins
  • frataxin