Bench to batch: advances in plant cell culture for producing useful products

Appl Microbiol Biotechnol. 2010 Feb;85(5):1339-51. doi: 10.1007/s00253-009-2354-4. Epub 2009 Dec 3.


Despite significant efforts over nearly 30 years, only a few products produced by in vitro plant cultures have been commercialized. Some new advances in culture methods and metabolic biochemistry have improved the useful potential of plant cell cultures. This review will provide references to recent relevant reviews along with a critical analysis of the latest improvements in plant cell culture, co-cultures, and disposable reactors for production of small secondary product molecules, transgenic proteins, and other products. Some case studies for specific products or production systems are used to illustrate principles.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biomass
  • Bioreactors*
  • Biotechnology / methods*
  • Cell Culture Techniques / methods*
  • Cells, Cultured
  • Coculture Techniques
  • Culture Media
  • Gene Expression Regulation, Plant
  • Plant Cells*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants / genetics
  • Plants / metabolism*
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / growth & development
  • Plants, Genetically Modified / metabolism
  • Protein Engineering
  • Recombinant Proteins / biosynthesis*


  • Culture Media
  • Plant Proteins
  • Recombinant Proteins