Absorption and effectiveness of orally administered low molecular weight collagen hydrolysate in rats

J Agric Food Chem. 2010 Jan 27;58(2):835-41. doi: 10.1021/jf9031487.


Collagen, a major extracellular matrix macromolecule, is widely used for biomedical purposes. We investigated the absorption mechanism of low molecular weight collagen hydrolysate (LMW-CH) and its effects on osteoporosis in rats. When administered to Wistar rats with either [(14)C]proline (Pro group) or glycyl-[(14)C]prolyl-hydroxyproline (CTp group), LMW-CH rapidly increased plasma radioactivity. LMW-CH was absorbed into the blood of Wistar rats in the peptide form. Glycyl-prolyl-hydroxyproline tripeptide remained in the plasma and accumulated in the kidney. In both groups, radioactivity was retained at a high level in the skin until 14 days after administration. Additionally, the administration of LMW-CH to ovariectomized stroke-prone spontaneously hypertensive rats increased the organic substance content and decreased the water content of the left femur. Our findings show that LMW-CH exerts a beneficial effect on osteoporosis by increasing the organic substance content of bone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorption
  • Administration, Oral
  • Animals
  • Chickens
  • Collagen / administration & dosage*
  • Collagen / blood
  • Collagen / chemistry
  • Collagen / pharmacokinetics*
  • Disease Models, Animal
  • Female
  • Femur / metabolism
  • Humans
  • Molecular Weight
  • Osteoporosis / drug therapy*
  • Osteoporosis / metabolism
  • Ovariectomy
  • Protein Hydrolysates / administration & dosage*
  • Protein Hydrolysates / blood
  • Protein Hydrolysates / chemistry
  • Protein Hydrolysates / pharmacokinetics*
  • Random Allocation
  • Rats
  • Rats, Wistar


  • Protein Hydrolysates
  • Collagen