Beneficial effect of docosahexanoic acid and lutein on retinal structural, metabolic, and functional abnormalities in diabetic rats

Curr Eye Res. 2009 Nov;34(11):928-38. doi: 10.3109/02713680903205238.


Purpose: To assess the effect of docosahexanoic acid (DHA) and lutein (both compounds with anti-inflammatory and antioxidant properties) on experimental diabetic retinopathy.

Methods: Male Wistar rats were studied: non-diabetic controls, untreated diabetic controls, and diabetic rats were treated with DHA and lutein or the combination of DHA + insulin and lutein + insulin for 12 weeks. Oxidative stress and inflammatory markers, apoptosis, and functional tests were studied to confirm biochemical and functional changes in the retina of diabetic rats. Malondialdehyde (MDA), glutathione concentrations (GSH), and glutathione peroxidase activity (GPx) were measured as oxidative stress markers. TUNEL assay and caspase-3 immunohistochemistry and electroretinogram were performed.

Results: Diabetes increases oxidative stress, nitrotyrosine concentrations, and apoptosis in the retina. At 12 weeks after onset of diabetes, total thickness of retinas of diabetic rats was significantly less than that in control rats. Specifically, the thickness of the outer and inner nuclear layers was reduced significantly in diabetic rats and demonstrated a loss of cells in the GCL. These retinal changes were avoided by the administration of insulin and DHA and lutein alone or in combination with insulin. Impairment of the electroretinogram (b-wave amplitude and latency time) was observed in diabetic rats. DHA and lutein prevented all these changes even under hyperglycemic conditions.

Conclusions: Lutein and DHA are capable of normalizing all the diabetes-induced biochemical, histological, and functional modifications. Specifically, the cell death mechanisms involved deserve further studies to allow the proposal as potential adjuvant therapies to help prevent vision loss in diabetic patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology
  • Antioxidants / pharmacology
  • Apoptosis
  • Biomarkers / metabolism
  • Blood Glucose / analysis
  • Caspase 3 / metabolism
  • Diabetes Mellitus, Experimental / metabolism
  • Diabetes Mellitus, Experimental / physiopathology
  • Diabetes Mellitus, Experimental / prevention & control*
  • Diabetic Retinopathy / metabolism
  • Diabetic Retinopathy / physiopathology
  • Diabetic Retinopathy / prevention & control*
  • Docosahexaenoic Acids / pharmacology*
  • Drug Therapy, Combination
  • Electroretinography
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique, Indirect
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • In Situ Nick-End Labeling
  • Insulin / pharmacology
  • Lutein / pharmacology*
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress
  • Rats
  • Rats, Wistar
  • Retina / drug effects*
  • Retina / physiopathology*
  • Tyrosine / analogs & derivatives
  • Tyrosine / metabolism


  • Anti-Inflammatory Agents, Non-Steroidal
  • Antioxidants
  • Biomarkers
  • Blood Glucose
  • Insulin
  • Docosahexaenoic Acids
  • 3-nitrotyrosine
  • Tyrosine
  • Malondialdehyde
  • Glutathione Peroxidase
  • Caspase 3
  • Glutathione
  • Lutein