Role of RNA structure in regulating pre-mRNA splicing

Trends Biochem Sci. 2010 Mar;35(3):169-78. doi: 10.1016/j.tibs.2009.10.004. Epub 2009 Dec 1.


Pre-mRNA splicing involves removing non-coding introns from RNA transcripts. It is carried out by the spliceosome, along with other auxiliary factors. In general, research in splicing has focused on the sequences within the pre-mRNA, without considering the structures that these sequences might form. We propose that the role of RNA structure deserves more consideration when thinking about splicing mechanisms. RNA structures can inhibit or aid binding of spliceosomal components to the pre-mRNA, or can increase splicing efficiency by bringing important sequences into close proximity. Recent reports have identified proteins and small molecules that can regulate splicing by modulating RNA structures, thereby expanding our knowledge of the mechanisms used to regulate splicing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Base Sequence
  • Exons
  • Humans
  • Introns
  • Molecular Sequence Data
  • Nucleic Acid Conformation*
  • RNA / chemistry*
  • RNA / genetics
  • RNA / metabolism
  • RNA Precursors / chemistry*
  • RNA Precursors / genetics
  • RNA Precursors / metabolism*
  • RNA Splicing*


  • RNA Precursors
  • RNA