Multipotent adult progenitor cell isolation and proliferation in cytokine and serum-free medium conditioned by rat B104 cells

Br J Haematol. 2010 Feb;148(3):441-4. doi: 10.1111/j.1365-2141.2009.07986.x. Epub 2009 Nov 30.

Abstract

This report describes the isolation of rodent multipotent adult progenitor cells (MAPCs) and proliferation of these cells in both standard medium and medium without exogenous serum or growth factors conditioned by the rat cell line B104. MAPCs have exacting requirements for their proliferation in vitro but once established proliferate rapidly at low seeding density, requiring almost daily passage and media exchange. Previously published methods for growth of MAPCs in vitro all used media supplemented with serum and growth factors, which adds considerable expense.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Cell Shape / drug effects
  • Cells, Cultured
  • Culture Media, Conditioned / pharmacology
  • Culture Media, Serum-Free / pharmacology
  • Cytokines / physiology
  • Male
  • Multipotent Stem Cells / cytology*
  • Multipotent Stem Cells / drug effects
  • Multipotent Stem Cells / physiology
  • Phenotype
  • Rats
  • Rats, Inbred Strains
  • Tumor Cells, Cultured

Substances

  • Culture Media, Conditioned
  • Culture Media, Serum-Free
  • Cytokines