Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Nov 27;326(5957):1268-71.
doi: 10.1126/science.1176951.

Transcriptome Complexity in a Genome-Reduced Bacterium

Affiliations

Transcriptome Complexity in a Genome-Reduced Bacterium

Marc Güell et al. Science. .

Abstract

To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms, Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.

Comment in

Similar articles

See all similar articles

Cited by 198 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback