Noncoding RNA of glutamine synthetase I modulates antibiotic production in Streptomyces coelicolor A3(2)

J Bacteriol. 2010 Feb;192(4):1160-4. doi: 10.1128/JB.01374-09. Epub 2009 Dec 4.

Abstract

Overexpression of antisense chromosomal cis-encoded noncoding RNAss (ncRNAs) in glutamine synthetase I resulted in a decrease in growth, protein synthesis, and antibiotic production in Streptomyces coelicolor. In addition, we predicted 3,597 cis-encoded ncRNAs and validated 13 of them experimentally, including several ncRNAs that are differentially expressed in bacterial hormone-defective mutants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / biosynthesis*
  • Blotting, Western
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial*
  • Glutamate-Ammonia Ligase / biosynthesis
  • Glutamate-Ammonia Ligase / genetics*
  • Models, Molecular
  • Nucleic Acid Conformation
  • RNA, Antisense / genetics
  • RNA, Antisense / metabolism*
  • RNA, Bacterial / genetics
  • RNA, Bacterial / metabolism*
  • RNA, Untranslated / genetics
  • RNA, Untranslated / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Streptomyces coelicolor / physiology*

Substances

  • Anti-Bacterial Agents
  • RNA, Antisense
  • RNA, Bacterial
  • RNA, Untranslated
  • glutamine synthetase I
  • Glutamate-Ammonia Ligase