Floxin, a resource for genetically engineering mouse ESCs

Nat Methods. 2010 Jan;7(1):50-2. doi: 10.1038/nmeth.1406. Epub 2009 Dec 6.


We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Attachment Sites, Microbiological / genetics
  • Base Sequence
  • Cell Line
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / metabolism*
  • Enhancer Elements, Genetic
  • Expressed Sequence Tags
  • Genetic Engineering / methods*
  • Genetic Markers
  • Genetic Vectors
  • Integrases / genetics
  • Integrases / metabolism
  • Mice
  • Mice, Transgenic
  • Mutation


  • Genetic Markers
  • Cre recombinase
  • Integrases